目的 研究简易库和冷藏库中牛膝理化指标的月动态变化规律。方法 牛膝在简易库和冷藏库中贮藏27个月,观察外观色泽,采用烘干法测定水分含量,采用高效液相色谱法(HPLC)测定β-蜕皮甾酮和5-羟甲基糠醛(5-HMF)的含量,采用相对温度积评价简易库与冷藏库温度差的累积,并进行理化指标的月动态变化规律的分析。结果 随着贮藏时间的延长,简易库中的牛膝色泽加深,质地变硬,而冷藏库中的牛膝色泽未见明显变化,质地较软;两库中牛膝的β-蜕皮甾酮的含量均逐渐下降,当贮藏到27个月时两库中牛膝的β-蜕皮甾酮含量低于《中国药典》规定的0.030%;5-HMF的含量均呈升高的趋势,且简易库中牛膝的5-HMF含量升高更多。结论 本实验首次提出了相对温度积的概念,并用于中药贮藏环节中化学成分的月动态变化规律研究。牛膝的理化指标随着贮藏月份的延长而变化。建议牛膝的贮藏期为2年。
Abstract
OBJECTIVE To study the monthly dynamics of physical and chemical indexes in Achyranthis Bidentatae Radix(ABR, the dried root of Achyranthes bidentata Bl.) stored in simple and cool warehouses. METHODS ABR was stored in simple and cool warehouses for 27 months. The color was observed. The water content was determined based on the drying method. The contents of β-ecdysone and 5-hydroxymethylfurfural (5-HMF) were determined by HPLC method. The accumulation of temperature difference between the simple and cool warehouses was evaluated with a relative temperature cumulation (RTC) method. The monthly dynamics of physical and chemical indexes of ABR was analyzed with RTC. RESULTS As the extension of storage time, the ABR stored in the simple warehouse showed deeper color and harder texture, but the ABR stored in the cool warehouse still had soft texture without significant color change. The contents of β-ecdysone in ABR stored in the two warehouses both gradually decreased and dropped to lower than the limit of 0.030% ruled by China Pharmacopoeia when being stored for up to 27 months. The contents of 5-HMF of ABR stored in the two warehouses both increased and were higher for the sample in the simple warehouse than that in the cool warehouse. CONCLUSION The concept of RTC is put forward and used to study the monthly dynamics of chemical constituents in traditional Chinese medicine during storage for the first time. The physical and chemical indexes of ABR varies during storage. Two years of storage time of ABR is suggested.
关键词
贮藏 /
牛膝 /
相对温度积 /
月动态变化 /
β-蜕皮甾酮 /
5-羟甲基糠醛
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Key words
storage /
Achyranthes bidentata /
relative temperature cumulation /
monthly dynamics /
β-ecdysone /
5-hydroxymethylfurfural
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中图分类号：
R97
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脚注
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基金
中医药行业科研专项资助(201407005);农业部现代农业产业技术体系建设专项资助项目(CARS-21)
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